Bacillus Subtilis Soluble Water 100CFU/G for feed As a Feed Additive, spores are living cells that are in a dormant state and can withstand the high temperature, high pressure, and acid-base environments during feed processing, making them stable and easy to store. Bacillus Subtilis exists in the form of spores in feed formulations and can enter the animal intestines in a live state for survival and reproduction, making it suitable for feed processing. Bacillus subtilis showed high survival rates in 90 ℃ high temperature, simulated gastric pH, pig bile salt test, and antibiotic susceptibility test; The preparation experiment of Bacillus subtilis preparation showed that the spore rate after drying and crushing treatment was greater than 98%. It can regulate the gut microbiota of livestock and poultry, increase the content of beneficial bacteria in the gut, and thus enhance the animal's resistance; It can be used in aquaculture to purify water quality and reduce harmful substances such as ammonia nitrogen and nitrite in water;
Application and Research in Livestock and Poultry Breeding
In the pig industry, piglets are prone to body weight loss, diarrhea, growth and development stagnation during the weaning process, and in severe cases, even lead to death. Bianlian et al. have shown that Bacillus subtilis inulin synbiotics can improve the growth performance and humoral immune function of weaned piglets. The Peo (1987) experiment confirmed that Bacillus subtilis can increase daily weight gain of growing pigs by 6% to 7%, and increase feed conversion rate by 3% to 4%.
In the poultry industry, Bacillus subtilis improves the composition of gut microbiota, enhances digestive enzyme activity, intestinal mucosal immune function, and enhances nutrient digestibility and feed absorption through equal pathways such as antioxidant water, ultimately promoting the growth of broilers, reducing the feed to egg ratio, and reducing the rate of broken or soft eggs. Research by Chen Caiwen and others has shown that Bacillus subtilis can significantly reduce the feed to weight ratio of chickens, increase the content of glucose and superoxide dismutase in the blood, reduce the content of malondialdehyde and alanine aminotransferase in the blood, increase the length of villi in the duodenum, jejunum, and ileum, and reduce their crypt depth. Tang Jiangwu test confirmed that Bacillus subtilis can germinate and remain in the intestines of experimental broiler chickens for more than 72 hours, and play a role in improving the activity of digestive enzymes in the intestines and improving the structure of the bacterial community. Zhai Ling et al. found that Bacillus subtilis can improve the antioxidant capacity of broilers, reduce oxidative stress, and thus improve their growth performance
In the aquaculture industry, Bacillus subtilis can secrete various enzymes, promote nutrient degradation in feed, and make aquatic animals more fully absorb and utilize feed; Degradation of residual feces, organic matter, etc. in the tank to improve water quality; It has a strong inhibitory effect on harmful microorganisms such as Vibrio, Escherichia coli, and rod-shaped viruses in aquatic products, effectively preventing diseases such as enteritis and gill rot in aquatic animals. The experimental results indicate that feeding Bacillus subtilis can enhance the body's ability to eliminate reactive oxygen species and free radicals, and increase the level of serum lysozyme activity. Research has shown that adding 2 to feed × 106-5 × 108CFU/g of Bacillus subtilis can promote the growth of bluefish species and reduce feed efficiency, but has no effect on fish body composition. Lei Aiying et al. isolated a strain of Bacillus subtilis and studied its purification effect on the water quality of South American white shrimp breeding ponds. The results showed that adding this bacterium to the ponds significantly reduced the COD value, nitrite, and H2S concentration of the pond water compared to the control pond, while the total alkalinity significantly increased. The Bacillus subtilis isolated by Zhang Fengfeng and others can quickly and effectively reduce the content of nitrate and nitrite in water, effectively reduce the pH value of the water, and purify the water.
In addition, Bacillus subtilis can reduce the occurrence of shrimp diseases, greatly increase shrimp production, thereby improving economic benefits, biological environmental protection, stimulating the development of immune organs in aquatic animals, and enhancing the body's immunity; Reduce the occurrence of shrimp diseases, significantly increase shrimp production, thereby improving economic benefits, purifying water quality, pollution-free, and residue free
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